In order to shift reaction eqref rxn:four to the still left ligands which preferentially bind to $ce Cu(I) ,$ should be added to the answer. As soon as the egg is produced, estradiol falls. It rises all over again in the mid-luteal stage after which drops again as your cycle https://c2dihydroceramided1802080134.is-blog.com/40563215/c2-dihydro-ceramide-d18-0-2-0-an-overview